Team:HokkaidoU Japan/Notebook/September15

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  • Observed results of yesterdays transformation
    • Transformation using heat shock went well
    • Electroporation transformation failed produce colonies
  • Did Colony PCR of yesterdays transformed colonies
  • Introduced colonies to L(+)Arabinose medium to check if it would show desired function
    • Check for results tomorrow


Result of yesterdays transformation

Transformation using heat shock went well and produced about 100 colonies. We chose 10 and did colony PCR to check if insert was correct. The insert was Arabinose promoter[http://partsregistry.org/Part:BBa_I0500 (BBa_I0500)] and GFP reporter[http://partsregistry.org/Part:BBa_E0840 (BBa_E0840)]. Vector used was [http://partsregistry.org/wiki/index.php?title=Part:pSB1C3 pSB1C3].

Combined length of insert with prefix, suffix and scar is estimated to be 2143bp. Electrophoresis picture under UV light

[Lamda/(Hindi3, EcoR1)]
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