"
Team:Cambridge/Notebook/10
From 2010.igem.org
Contents |
Monday
To our pleasant surprise our plates of P. pyralis in pSB1C3, L. cruciata in pSB1C3 and L. cruciata with pBAD in SB1C3 had all grown successfully over the weekend. Only P. pyralis with pBAD had not grown, which Hannah and Emily (who was back from holiday) repeated that experiment today. The thioesterase plates had also grown over the weekend as well, which was very exciting! Ben set up a plate reader experiment going overnight to test whether L. cruciata luciferase worked and Theo took very pretty pictures of light bulbs glowing with bacteria!
In trying to be organised, we've also grown up broths of the original DNA2.0 plasmids and the products of the plates to make glycerol stocks tomorrow.
Tuesday
Wednesday
luorescent proteins onto the two operons, continuing Tuedays experiment and plated these out.
Thursday
Friday
Saturday
Sunday
