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Revision as of 15:35, 12 September 2010 by
Imb10
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PCR Result
9/6 ~ 9/9 PCR Result
PCR composition
Materials
Amount
Template
100pmole
Primer A
50pmole
Primer B
50pmole
dNTP (dNTP mixture 2.5mM TaKaRa)
2ul
Taq (Pfu Turbo® DNA Polymerase 2.5U/ μl STRATAGENE)
0.25ul
10x buffer (10x cloned Pfu Reaction buffer)
5ul
DW (3rd sterile water)
rest
Total
50ul
exp 1
Segment
Annealing Temperature
Result
a
STAT1
53*C
-
-
b
V_STAT1
53*C
-
-
c
Fusion Antibody receptor
53*C
-
-
d
Signal peptide
56*C
-
-
e
Ig-like
56*C
-
-
f
Signal Peptide + Ig-like
56*C
-
-
g
Antibody
56*C
-
-
h
V_Fusion Antibody Receptor
53*C
-
-
i
B_FGFR1
56*C
-
-
j
VB_FGFR1
56*C
-
-
exp 2