Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/08

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2010/09/08

PCR

<Member>

Hitomi


<Sample>

  • 5
  • 1+2(ligation 2010/08/31)
  • 4+5(ligation 2010/08/31)
  • 7+8(ligation 2010/09/06)


<Protocol>

See Protocol2 (https://2010.igem.org/Team:Tokyo_Metroporitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/)


  • Tube(temperature in annealing)
1.(71.0,70.5,70.0℃)
2.(72.0,71.5℃)
3.(70.0℃)
4.(70.0℃)
Total 12 tubes.
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