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Team:LMU-Munich/Notebook/Protocols/17 Special thermal cycler programs/Touch down phusion
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Revision as of 13:09, 10 September 2010 by MengzheWang (Talk | contribs)
PCR program: Touch-Down with Phusion
98°C
1 min
98°C
10 sec
59°C
20 sec
73°C
20 sec
return to step 2 for 2 cycles
98°C
10 sec
57°C
20 sec
73°C
20 sec
return to step 6 for 2 cycles
98°C
10 sec
55°C
20 sec
73°C
20 sec
return to step 10 for 2 cycles
98°C
10 sec
53°C
20 sec
73°C
20 sec
return to step 14 for 2 cycles
98°C
10 sec
51°C
20 sec
73°C
20 sec
return to step 18 for 2 cycles
98°C
10 sec
49°C
20 sec
73°C
20 sec
return to step 22 for 2 cycles
95°C
30 sec
53°C
30 sec
72°C
30 sec
return to step 26 for 29 cycles
73°C
10 min
12°C
forever
