Team:Tsinghua/Notebook/7 September 2010
From 2010.igem.org
YX's part
Repeated the experiments the day before. Doubled the antibiotic dose. The result was fine.
Enzymatic digested the segments from the vector with DraIII for 2 hours.
Ran an agarose gel and recycled the cutout sequence.
Did the ligation reaction with Takara Solution I at 16 centigrade.