Team:UNIPV-Pavia/Calendar/July

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JULY



Bio-Lab activity proceeds!

Select the day you are interested in to find out the details!

DATEACTIVITY
July, 1st

19° Bio-Lab -I7-3,4 and 5 and I8-3, 4 and 5 were prepared for TECAN test.
Of the plates incubated yesterday, a colony was picked and grown in 1ml LB (+ antibiotic) for 6 hours at 37°C 220 rpm. Other parts were received from Anderson Lab.

July, 2nd

20° Bio-Lab - TECAN test provided encouraging results, showing that I7-3, I7-5, I8-4 and I8-5 produced GFP.
Other culture (I7-1, I7-2, I7-4, I8-1, I8-2, I8-3) did not produce GFP, so they were thrown away. A furhter screening were performed on I7-3, I7-5, I8-4, I8-5 and this time all the clones are OK . Inoculum of <partinfo>BBa_J72007</partinfo> and <partinfo>BBa_J72013</partinfo> from LB agar plates.A glycerol stock was prepared for each culture.
Inoculum of <partinfo>BBa_J72008</partinfo> from LB agar plate grown at 30°C 220 rpm overnight.

JULY: WEEK 2
July, 5th21° Bio-Lab
July, 6th22° Bio-Lab - Lorenzo, Susanna Z., Federica, Nicolò.
July, 7th23° Bio-Lab - Lorenzo, Susanna Z., Sara, Nicolò.
11° Meeting - Updating about Bio-Lab activity.
July, 8th24° Bio-Lab - Giacomo, Alessandro, Federica. Transformation of <partinfo>BBa_J23118</partinfo> in our home-made competent strains
  • MG1655
  • BW25141
  • BW25142
  • BW53474
  • DH5alpha (as control)
to check transformation efficiency.
July, 9th25° Bio-Lab - Giacomo, Nicolò. All plates showed red colonies, so we calculated the efficiency of the strains.
JULY: WEEK 3
July, 12th26° Bio-Lab -
July, 13th27° Bio-Lab -
July, 14th28° Bio-Lab -
July, 15th29° Bio-Lab -
July, 16th30° Bio-Lab -
JULY: WEEK 4
July, 19th31° Bio-Lab - Susy Z. Nicolò, Federica, Matteo M.
July, 20th32° Bio-Lab - Susy Z., Nicolò, Federica, Riccardo.
July, 21st33° Bio-Lab - Susy Z, Nicolò, Sara, Alessandro
July, 22nd34° Bio-Lab - Susy Z, Nicolò, Federica, Riccardo, Giacomo.
July, 23rd35° Bio-Lab - Susy Z. Nicolò, Federica, Alessandro, Giacomo
July, 24th36° Bio-Lab -
July, 25th37° Bio-Lab -
JULY: WEEK 5
July, 26th38° Bio-Lab -
12° Meeting - Updating about Bio-Lab activity and organization of our trip to Boston!
July, 27th39° Bio-Lab -
July, 28th40° Bio-Lab -
July, 29th41° Bio-Lab -
July, 30th42° Bio-Lab -
July, 31th43° Bio-Lab -