Team:UC Davis/protocols/digestion.html

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Digestion

Materials

You will need:

  • Sterilized milliQ water
  • BSA
  • NEB buffers 1,2,3,and/or 4
  • Miniprepped sample or plasmid you wish to cut
  • NEB digestion enzymes (EcoRI, SpeI, PstI, NheI, and/or XbaI)

Procedure

  • Add 22μL of milliQ water.

Purpose

To cut DNA at the designated places.

References

We would like to take a moment to thank all of our sponsors for their very generous donations, as we could not have done this without your help!

We would also like to thank and acknowledge:
Our Advisors
Marc Facciotti
Ilias Tagkopoulos
Technical Guidance
David Larsen
Andrew Yao
Visiting iGEMer
Jia Li of Zhejiang University (TEAM ZJU-China)
cI Promoter Screen
Drew Endy - Stanford
Thomas Schneider - NIH
Want to sponsor us? Send an email to mtfacciotti@ucdavis.edu to discuss various ways you can help! :)

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