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Team:Kyoto/Notebook
From 2010.igem.org
LastModified: 2010-9-6
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Notebook
Tuesday, July 20
By: Wataru, Tomo, Yuki, Kazuya, Ken, Makoto
1. Solubilization of antibiotics.
- for Ampicillin(Amp): add 1.0g Amp to 20ml MilliQ (final concentration is 50mg/ml).
- for Kanamycin(kan): add 0.5g Kan to 10ml MilliQ (final concentration is 50mg/ml).
- Dispense 1.1ml of the solution into 1.5ml tubes.
- Store in the freezer (-20℃).
2. Making plates for LB (Amp+) and LB (Kan+).
3. Transformation of iGEM Parts.
| Name | Well | Sample (µl) | Competent Cells (µl) | Total (µl) | Plate | Incubation | Result |
|---|---|---|---|---|---|---|---|
| <partinfo>J23100</partinfo> | 1-18-C | 1 | 20 | 21 | LB (Amp+) | At 37℃ 7/20 20:50 - 7/21 17:00 | ○ |
| <partinfo>J23105</partinfo> | 1-18-M | 1 | 20 | 21 | ○ | ||
| <partinfo>J23116</partinfo> | 1-20-M | 1 | 20 | 21 | ○ | ||
| <partinfo>R0011</partinfo> | 1-6-G | 1 | 20 | 21 | ○ | ||
| <partinfo>E0840</partinfo> | 1-12-O | 1 | 20 | 21 | ○ | ||
| <partinfo>J06702</partinfo> | 2-8-E | 1 | 20 | 21 | ○ | ||
| <partinfo>pSB4K5</partinfo> | 1-5-G | 1 | 20 | 21 | × | ||
| <partinfo>B0015</partinfo> | 1-23-L | 1 | 20 | 21 | LB (Kanamycin+) | × |
- "1-18-C" means well 18C in [http://partsregistry.org/Help:Spring_2010_DNA_distribution Spring 2010 DNA Distribution Kit] Plate 1.
- Discussion
- A vector of "pSB4K5" is Kanamycin-resistance, however, we plated it to LB plate (Ampicillin+).
- And We didn't pre-culture "B0015" despite its vector is Kanamycin-resistance.
- So, it was predicted that we will fail the transformation of "pSB4K5" and "B0015".
