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From 2010.igem.org

Let's go people!!!!!
SOS repair system & your ideas!!

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I found Aberdeen_Scotland team in 2009 igem can be a good guide to our curcuit.
This team used lacI forms complex with IPTG.
Cl and Cl operator can be used as a switch.

--Sadesabin 06:04, 14 August 2010 (UTC)

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https://2009.igem.org/Team:Aberdeen_Scotland/WetLab/lacilatch
https://2009.igem.org/Team:Aberdeen_Scotland/Project#Introduction
이거슨 Averdeen 팀의 프로젝트 개요!

--Needks 07:10, 14 August 2010 (UTC)

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IPTG, LacI and CI are related
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC139174/pdf/pq2602016516.pdf

This file also note that.

--Sadesabin 06:32, 16 August 2010 (UTC)

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• TetR structure

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1197418/bin/zmr0020520860002.jpg

tetR-controlled activation regulation

• http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1197418/

When TetR binds to tetR operator, it makes the promoter attached to operator work
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1197418/bin/zmr0020520860003.jpg
Binding of TetR to its operator site. A) tetR operator and contact regions. The tetR operator is a palindromic sequence. Horizontal bars show nucleotides contacted by each monomer of the TetR dimer. B) Interaction of TetR residues with specific nucleotides (arrows) and phosphate backbone (blue lines) in the operator region. The amino acids involved in DNA binding extend from residues 27 to 48. Contacts established with the operator were confirmed by footprint assays, by analysis of TetR mutants, and by crystallographic studies (29, 30, 159, 266, 366). C) Representation of each homodimer bound to the tet operator in a double-helix representation. (Adapted from Orth et al. [288] with permission of the publisher.)

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1197418/bin/zmr002052086008a.jpg

--Needks 14:35, 4 September 2010 (UTC)

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