Team:Tsinghua/Notebook/26 August 2010

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Module I, DT and Fan's part:

Run a colony PCR with universal primers. Amplify the bacteria at the same time.

PCR system (SuperMix):

 H2O	        8μl
 primer1	1μl
 primer2	1μl
 template	Some
 SuperMix	10μl
 Total	        20μl

result

10-8-26.jpg

The PEMKC 3-5 are positive. We’ll wait for the sequence result to ensure.