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From 2010.igem.org

Module I, DT and Fan's part:

Measure the concentration of the PCR products.

Gene Concentration(μg/ml) OD 260/280 eGFP 95.9 1.73 mCherry 61.2 1.74 Kan 160.5 1.67 Chlr 167.5 1.74

Digest the four fragments with follow system at 37℃ for 10 hours.

Digestion system:

eGFP:

 H2O	23.5μl
 10×buffer	5μl
 eGFP	20μl
 HindIII	1.5μl
 Total	50μl

mCherry:

 H2O	11.5μl
 10×buffer	5μl
 mCherry	32μl
 HindIII	1.5μl
 Total	50μl

Kan:

 H2O	31μl
 10×buffer	5μl
 Kan	12.5μl
 KpnI	1.5μl
 Total	50μl

Chlr:

 H2O	31.5μl
 10×buffer	5μl
 Chlr	12μl
 KpnI	1.5μl
 Total	50μl

Productions purify and measure the concentration.

Gene Concentration(μg/ml) OD 260/280 eGFP 38.4 1.66 mCherry 28.8 1.86 Kan 43.7 2.11 Chlr 31.9 1.77