Team:EPF Lausanne/Notebook/week5
From 2010.igem.org
Contents |
Week 5
09-08-2010
Biobrick |
Asaia
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10-08-2010
ImmunotoxineWe'll made some GLY stock of E-coli containing the immunotoxine plasmid (shiva). So today we transform E-coli with the plasmid and we plated on Amp overnight. |
BiobrickWe try to begin to construc two new biobrick today but we made mistake. We'll start again tomorrow. |
11-08-2010
ImmunotoxineWe've pick up some colony of transformed cells from yesterday and make liquid culutre of them. Tomorrow we'll make some GLY stock and a gel to control. With this stock we can begin to work safely with the immunotoxine. |
AsaiaTo test our asaia origin sequence, we take a BV with (1) asaia origin (2) Amp resistance (3) death casette and we will throw away the death casette and just transform asaia with this simpel plasmid. Today we just make a liquid culture from a GLY stock with E-coli containing this plasmid. |
BiobricksWe begin to construc a new Biobrick containing (1) Base Vector (2) Strong or Weak promotor (3) RBS. Today we made digestion, ligation and E-coli transformation. Plated overnight on Amp. |
DrosophilePersistence of bacteria in drosophilia's gut. We infected (fed) flies with Asaia. We also infected flies with persistant or pathogenic bacteria (Pseudomonas in control experiment). Then we crushed, diluted and plated infected flies after 3h hours from infection. We will plate flies after 24 and 48 hours as well and see how many colonies appear on the plates. We hope to get an idea of the persistency of Asaia in the gut of Drosophila. |
12-08-2010
Drosophila
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BiobrickDigestion of Immunotoxine Other jobs failed and we discovered we have to restart many digestion/ligation Digestion C3+Promotor(weak/strong)+RBS Digestion C3+Promotor(w/s)+RBS+Immuno |
13-08-2010
Drosophilae
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Asaia
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BiobrickDigestion of Immunotoxine Other jobs failed and we discovered we have to restart many digestion/ligation Digestion C3+Promotor(weak/strong)+RBS Digestion C3+Promotor(w/s)+RBS+Immuno |
14-08-2010
BioBricksToday we came to check our culture of E-coli transformed with the plasmid C3 + Promotor + RBS (+ Immunotoxine) but no plate have grown!! |
AsaiaWe also transformed E-coli 3.1 with the Base Vector (BBa_I51020) + Asaia Origin + Resistance. |
15-08-2010
AsaiaToday we just come and check our transformed cells of yesterday. We have colonies on two plates. Work continue tomorrow. |