Team:Stockholm/28 August 2010

From 2010.igem.org

Revision as of 10:43, 30 August 2010 by AndreasConstantinou (Talk | contribs)


Andreas

Gel extraction

No measurable DNA conc. for "Extr. Dig SOD E+A"
DNA concentrations
Sample Conc [ng/μl] A260/A280
Extr. N-CPP 40.91 1.69
Extr. Dig SOD E+A
Pur. Dig pMA.His E+N 36.02 1.80

From 27/8
Purified DNA from excised samples "Extr. Dig. SOD E+A" and "Extr. N-CPP cluster" using the E.Z.N.A. Gel extraction kit; procedures according to provided protocol.

Elution volume: 30 μl (eluted twice to increase DNA yield)

  • Extr. N-CPP
  • Extr. Dig SOD E+A

DNA purification of "Dig. pMA.His (E+N)"

From 27/8
DNA clean-up using the E.Z.N.A. Gel Extraction kit, following procedures for DNA purification.

Elution volume: 30 μl (eluted twice to increase DNA yield)

  • Pur. Dig. pMA His E+N