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From 2010.igem.org

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Wet-lab members are working on several proteins. The idea behind why we chose these proteins was somehow vague, because of low amount of information we had on our wiki page. In this section we try to explain the reasons and ideas behind choosing those specific proteins.

As a starting point, we began with the article [http://www.ncbi.nlm.nih.gov/pubmed/18426409 Strömberg S et al. (2008)]. In this paper they identified some 859 genes as differentially regulated genes in melanocytes. These genes can be classified in several groups based on their possible role. These groups are:

1. Developing melanocytes
2. Melanin synthesis and delivering it using melanosomes
3. Cell adhesion
4. Antigen presenting

out of those genes, it seemed that [http://www.ncbi.nlm.nih.gov/gene/4286 MITF] is one of the most effecting and effected genes in vitiligo disease. This was the first block of the map. to be continued...

[http://www.ncbi.nlm.nih.gov/gene/5077 PAX3]

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We start by looking at several databases how the PPI map should look like for the proteins of interest, from there we can make statements about predictions or make prove of concept. One of the main articles which looked into transcriptional profile in melanocytes from vitiligo patients is Strömberg S et al. Pigment Cell Melanoma Res. (2008). For the first phase of interaction map, focuse will be on MITF. For this case, I chose several genes which are differentially regulated from Strömberg S et al. Pigment Cell Melanoma Res. (2008). foxd3, pax3 and tyrp1 are chosen (Notes from August 6 and August 5).