From 2010.igem.org
Building Parts
To create parts that we want to use in our finished kit, we first started by finding a standard plasmid in which we could put each part in to amplify it.
May
S | M | T | W | R | F | S
|
| | | | | | 1
|
2 | 3 | 4 | 5 | 6 | 7 | 8
|
10 | 11 | 12 | 13 | 14 | 15 | 16
|
17 | 18 | 19 | 20 | 21 | 22 | 23
|
24 | 25 | 26 | 27 | 28 | 29 | 30
|
31 | | | | | |
|
June
S | M | T | W | R | F | S
|
| 1 | 2 | 3 | 4 | 5 | 6
|
7 | 8 | 9 | 10 | 11 | 12 | 13
|
14 | 15 | 16 | 17 | 18 | 19 | 20
|
21 | 22 | 23 | 24 | 25 | 26 | 27
|
28 | 29 | 30 | | | |
|
July
S | M | T | W | R | F | S
|
| | | 1 | 2 | 3 | 4
|
5 | 6 | 7 | 8 | 9 | 10 | 11
|
12 | 13 | 14 | 15 | 16 | 17 | 18
|
19 | 20 | 21 | 22 | 23 | 24 | 25
|
26 | 27 | 28 | 29 | 30 | 31 |
|
August
S | M | T | W | R | F | S
|
1 | 2 | 3 | 4 | 5 | 6 | 7
|
8 | 9 | 10 | 11 | 12 | 13 | 14
|
15 | 16 | 17 | 18 | 19 | 20 | 21
|
22 | 23 | 24 | 25 | 26 | 27 | 28
|
29 | 30 | 31 | | | |
|
May 10, 2010
PCR amplified a KanR cassette from p1003 with primers that contained either the A and B' ends or the A' and B ends.
Image of Gel