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Team:Cambridge/LabBook/Week6
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42. Experiment: Transforming TOP10 with pBAD promoter (Hannah)
Took plasmid from Shuna, followed protocol from earlier, growing up stock to make more plasmid so we can PCR from it later.
Plated out on Kanomycin plates
43. Experiment: Plasmid extraction - pHK555 + LuxR from registry (Hannah)
Followed Qiagen miniprep protocol
| Plasmid | Concentration (ng/μl) |
|---|---|
| pHK555 | 20.9 |
| 17.3 | |
| LuxR | 43.9 |
| 89.2 |
