Lab work

BioBrick stocks

Today we performed a Qiagen Mini-prep plasmid isolation of the vectors we grew overnight. We saved 3 mL of the bacterial cells to make -80 °C stocks.

The following plasmid concentrations were obtained:

BioBrick	Concentration (ng/μL)
pSB3T5	266.0
pSB1T3	414.0
pSB1C3	284.7
pSB3C5	329.3
pSB1K3	270.1
pSB1A3	271.9
I13401	232.1

Ramon & Hugo in the lab

Thias doing labwork

Characterization of Anderson RBS sequences

Next to that we also started digesting the first parts we will be characterizing.

Strain Characterization

By Hugo

I prepared the cell bank according to the following procedure:

Spin 5 ml of the overnight culture
Add 500 ul of LB medium
Add 500 ul of 80% glycerol
Store the tube at -80ºC

Now, we have a cell bank ready to use!!!

Spring Workshop

Almost the whole team left for the iGEM spring workshop in Évry, Paris. The drive for some of us was a bit laborious (traffic jams at the middle of the night/closed roads etc.), and others actually watched the World Cup soccer match... But in the end we all arrived at the hotel in Évry little before 2 AM, Saturday morning, and got some well deserved sleep.