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From 2010.igem.org

More media

Today camera crew #2 came by from Nanopodium, a platform for exchanging thoughts, ideas, opinions and best practices on nanotechnology. They are making a movie about our project for the Nano Video Hunt. This is a campaign started with Hyves were young people are challenged to post videos about their views on nanotechnology.

For more information look at the following websites:

[http://www.nanovideohunt.nl www.nanovideohunt.nl]

[http://www.nanovideohunt.hyves.nl www.nanovideohunt.hyves.nl]

[http://www.nanopodium.nl www.nanopodium.nl]

Lab work

By Hugo

I made some time for doing some transformations from the Biobrick plates, I will give you the list of the biobricks that I tried to transform.

Procedure:

• Add 1 ul of DNA to 33 ul of competent cells
• Put the tubes on ice for 10 min
• Warm the tubes in a water bath at 37º C for 5 min.
• Add 250 ul of LB medium
• Incubate the cells 1 hour at 37ºC, shake at 160 rpm
• Plate 200 ul over selective medium

#	Code	Resistance marker	Function
1	J61100	Amp	RBS Anderson family
2	J61101	Amp	RBS Anderson family
3	J61107	Amp	RBS Anderson family
4	J61117	Amp	RBS Anderson family
5	J61127	Amp	RBS Anderson family
6	B0034	Amp	RBS
7	J23109	Amp	Promoter Anderson family
8	B0032	Amp	RBS Anderson family
9	R0011	Amp	LacI promoter
10	B0015	Amp, Kan	Double terminator
11	R0010	Amp	LacI
12	E0422	Amp	YFP generator
13	E0240	Amp	GFP generator
14	E0040	Amp	GFP
15	I13401	Amp	GFP + Double terminator