Team:Tokyo Metropolitan/Notebook/Pattern/2010/08/25

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Experiment5：Extraction of a gel

＜Member＞Mariko, rsk, hitomi

＜Materials＞
・Thermostable β-Agarase 6 units

・Agarose gel(after DNA gel electrophoresis)

・Dropped water 400ml


＜Protocol＞
1 Prepared 400ml solution to contain Yeast extract 2.0g, Peptone 2.0g, Glucose 40.0g, NaCl 2.0g and Agerose 6.0g.

2 Divided up 400ml solution into two Erlenmeyer flasks, and sealed with aluminum. Afterwards , started autoclave(121℃,20min)

3 After finished autoclave, in cleanbench, added 0.2ml Ampcillm to each of the solutions. Afterwards, divided solutions into twenty Petri dishes.

4 Cooled and dried divided solutions in Petri dishes. After Cooling and drying well, kept these in refrigerator.

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