Pathway Notebook
Contents
8-02-2010
8-03-2010
Some test text in bold
We created following tests:
8-04-2010
Example of a table
| header 1
| header 2
| header 3
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| row 1, cell 1
| row 1, cell 2
| row 1, cell 3
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| row 2, cell 1
| row 2, cell 2
| row 2, cell 3
|
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8-24-2010
making competent cells
with Benny's protocoll & buffers
- grow the culture to an OD 600 of ~ 0,3
-> our cells: OD 600 = 0,256
- centrifuge for 10 min at 4°C by 3000rpm
- resuspend the pellet in buffer 1
-> we used 400 µl
- centrifuge for 10 min at 4°C by 2500rpm
- resuspend the pellet in less buffer 1 than before
-> we used 200 µl
- add buffer 2 in a ratio of 1:10 = buffer 2:buffer 1
-> we added 20µl of buffer 2
- incubate on ice for 10 min
-> as we probably used too little buffer, we added another 200 µl of buffer 1 and 20µl of buffer 2
- aliquote the suspension and shockfreeze it at -80°C
-> we put 50 µl in each eppendorf
test-transformation
in order to see whether the sells will work
- done with protocoll https://2010.igem.org/Team:LMU-Munich/Notebook/Protocols/3_Transformation
-> we tested with the following DNA:
- 1. K098200 (biobrick) [Amp., 5 µl]
- 2. pUC [Amp., 1µl]
-> as the cells are probably in a to high concentration, we thined them down with saline to an end-concentration of 10 -2
8-25-2010
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8-26-2010
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8-27-2010
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8-28-2010
weekend
8-29-2010
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8-30-2010
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8-31-2010
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9-01-2010
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9-02-2010
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9-05-2010
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9-04-2010
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9-05-2010
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9-06-2010
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9-07-2010
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9-08-2010
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9-09-2010
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9-10-2010
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9-11-2010
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9-12-2010
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9-13-2010
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9-14-2010
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9-15-2010
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9-16-2010
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9-17-2010
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9-18-2010
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9-19-2010
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9-20-2010
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9-21-2010
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9-22-2010
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9-23-2010
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9-24-2010
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9-25-2010
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9-26-2010
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9-27-2010
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9-28-2010
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9-29-2010
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9-30-2010
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10-01-2010
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10-02-2010
weekend
10-03-2010
weekend
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