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From 2010.igem.org

Contents 1 August 24th (bambi75) 1.1 Agarose Gels Electro-phoresis 1.1.1 member 1.1.2 Materials,Procedure 1.2 Medium making 1.2.1 member 1.2.2 Materials , Procedure 1.3 PCR 1.3.1 member 1.3.2 materials 1.3.3 Procedure 1.4 Restriction enzyme digestion 1.4.1 member 1.4.2 Materials 1.4.3 Procedure 1.5 Transformation 1.5.1 member 1.5.2 Materials , Procedure

August 24th (bambi75)

Agarose Gels Electro-phoresis

member

NEX , bambi75 and watachin

Materials,Procedure

Same protocol on August 16th. PCR Product of insert are bcsA and bcsC.

Medium making

member

easily and naoto

Materials , Procedure

Same protocol on August 10th.

PCR

member

NEX , bambi75 and watachin

materials

• sterilized water 213μl
• Ex taq buffer 30μl
• dNTP 24μl
• Ex taq 3μl
• K12bcsA sense 15μl
• K12bcsA antisense 15μl

Procedure

Same on August 23th.

Restriction enzyme digestion

member

Same above

Materials

• PCR product
• m buffer 30μl
• BSA 30μl
• Xba1 6μl
• SPE1 6μl

Procedure

Same on August 13th.

Transformation

member

Same above

Materials , Procedure

Same on August 17th.