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Electrophoretic run (Practice)

Time: From 9:00 to 18:00
[Member] Iwaki, Fukuyama, Takeuchi, Usui, Adachi
[Details of Experiment]
(1)We made 1% Agarose Gel by using Agarose 0.2g and TEA 20μL.
(2)We mixed DNA 5μL, Marker 1μL and Loading Buffer 1μL.
We applied each 5μL of them to gel.We applied Marker 5μL to the far left lane.
(3) 100V, 30min electrophoresed
(4)Gel soaked in EtBr for 20min.
(5) Band Shooting.