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From 2010.igem.org

Contents 1 Hassan 2 Mimmi 2.1 pMA 2.1.1 Primer dilution 2.2 pMA/pEX 2.2.1 verification PCR

Hassan

midnight update, after a fast recovery from sickness!

Mimmi

pMA

Primer dilution

pMA_VF		91µl sH2O --> 100µM		10µl + 90µl sH2O --> 10µM
pMA_VR		91µl sH2O --> 100µM		10µl + 90µl sH2O --> 10µM

pMA/pEX

verification PCR

		pEX	pMA
Mix	(µl)	X6	X3		primers		conditions
sH2O	22.5	135	67.5		pEX_VF		time	°C
F primer	1	6	3		pEX_VR		2m	94
R primer	1	6	3		pMA_VF		30s	94	)
DNA	0.5	5X0.5	2X0.5		pMA_VR		30s	60	> 30 cycles
tot	25µl	250µl	75µl				2m30s	72	)
							10m	72
							oo	10