PCR and mutagenisis of the digestion sites
PCR1a: replication of Pdu D with mutagenesis [ ]
expected product size: bp
PCR1b: replication of Pdu D with mutagenesis [ ]
expected product size: bp
PCR2: Joining PCR of PduD [ ]
expected product size: bp
PCR3a: replication of Pdu C with mutagenesis [ ]
expected product size: bp
PCR3b: replication of Pdu C with mutagenesis [ ]
expected product size: bp
PCR4: Joining PCR of Pdu C [ ]
expected product size: bp
PCR5: replication of AurF to be joined with Pdu D [ ]
expected product size: bp
PCR6a: replication of LctO with mutagenesis [ ]
expected product size: bp
PCR6b: replication of LctO with mutagenesis [ ]
expected product size: bp
PCR7: Joining PCR of LctO [ ]
expected product size: bp
PCR8a: replication of LctO with mutagenesis [ ]
expected product size: bp
PCR8b: replication of LctO with mutagenesis [ ]
expected product size: bp
PCR9: Joining PCR of LctO [ ]
expected product size: bp
PCR10: Joining PCR of AurF and PduD [ ]
expected product size: bp
PCR11: Joining PCR of LctO and PduC [ ]
expected product size: bp
PCR12a: replication of the Pdu-Operon with mutagenesis [ ]
expected product size: bp
PCR12b: replication of the Pdu-Operon with mutagenesis [ ]
expected product size: bp
PCR13: Joining PCR of the Pdu-Operon [ ]
expected product size: bp
PCR14a: replication of the Pdu-Operon with mutagenesis [ ]
expected product size: bp
PCR14b: replication of the Pdu-Operon with mutagenesis [ ]
expected product size: bp
PCR15: Joining PCR of the Pdu-Operon [ ]
expected product size: bp
PCR16a: replication of the Pdu-Operon with mutagenesis [ ]
expected product size: bp
PCR16b: replication of the Pdu-Operon with mutagenesis [ ]
expected product size: bp
PCR17: Joining PCR of the Pdu-Operon [ ]
expected product size: bp
In Vitro testing of the chemical kinetics with the enzymes
Transforming the construct in bacteria
Testing the system
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expected product size: bp
