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From 2010.igem.org

Today, we learned the dimensions needed for a Polymerase Chain Reaction (PCR) Master Mix (which can be found in Lab Protocols) and attempted a PCR of our own to multiply the DNA. We used plasmids R0040 123502 (Red), R0040 GFP C2 (Blue) and R0040 GFP C1 (Yellow). In addition to this, we used agarose gel electrophoresis to determine whether or not the DNA was indeed present.