Aim 1: DNA reproduction+PCR
Colourcode for the primers: Annealing part, mutagenised part, other sequences integrated into primer
PCR1: replication of the Tet-inducible CMV promotor [X]
Primer used: 1TreF 2TreR
PCR4a: replication human bak with mutagenesis (Pst1) [ ]
Primer used: 7BakF 8BakMutPR
PCR4b: replication human bak with mutagenesis (Pst1) [ ]
Primer used: 9BakMutPF 10BakR
PCR5: joining PCR of human bak [ ]
Primer used: 7BakF 10BakR
PCR6: replication of the SV40-polyadenylation site [X]
Primer used: 11PAF 12PAR
PCR9: replication of eGFP with attP in primer [ ]
Primer used: 20eGFPattPF 21eGFPR
PCR10: Replikation of PhiC31o [ ]
Primer used: 22PC31oF 23PC31oR
Note: for attB two Primers ( 18attBF 19attBR) used.
Aim 2: Assembling Biobricks
Aim 3: Testing products
Aim 4: Testing system
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