From 2010.igem.org
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Aim 1: DNA reproduction+PCR
PCR1: replication of the Tet-inducible CMV promotor [X]
Primer used: 1TreF 2TreR
PCR4a: replication human bak with mutagenisis (Pst1) [ ]
Primer used: 7BakF 8BakMutPR
PCR4b: replication human bak with mutagenisis (Pst1) [ ]
Primer used: 9BakMutPF 10BakR
PCR5: joining PCR of human bak [ ]
Primer used: 7BakF 10BakR
PCR6: replication of the SV40-polyadenylation site [X]
Primer used: 11PAF 12PAR
Aim 2: Assembling Biobricks
Aim 3: Testing products
Aim 4: Testing system
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