Team:Cambridge/biobrickingoligosfirefly

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1 ttcgctaaggatgatttctg gaattcgcggccgcttctag ag Tm:70.14 dG:1.54

2 ttgcccgtttttttgccgga ctgcagcggccgctactagta Tm:68.73 dG:-0.26

3 tactagtagcggccgctgcag Tm:68.73 dG:0.57

4 ctctagaagcggccgcgaatt Tm:70.55 dG:0.67

5 gaattcgcggccgcttctagagCGCCGGTGTGAAAGTTAAGCT (borrows some of the PP.LRE to get a close melting temp) Tm:67.53 dG:-0.39

6 ctgcagcggccgctactagtaTTACAGCTTAACTTTCACACCGGC Tm:67.18 dG:0.41

7 GCCGGTGTGAAAGTTAAGCTGTAAtactagtagcggccgctgcag Tm:67.18 dG:0.57

8 AGCTTAACTTTCACACCGGCGctctagaagcggccgcgaattc Tm:67.53 dG:-0.54

9 gaattcgcggccgcttctagagGGGTGACCGTGTTAAGCTGT Tm: 64.34 dG:0.46

10 ctgcagcggccgctactagtaTTACAGCTTAACACGGTCACCC Tm:65.21 dG:0.41

11 GGGTGACCGTGTTAAGCTGTAAtactagtagcggccgctgcag Tm:65.21 dG:0.57

12 ACAGCTTAACACGGTCACCCctctagaagcggccgcgaattc Tm: 64.34 dG: 0.67