Public Safety
Our strain of E. Coli is not pathogenic, so no epidemic disease wiping the population will occur as a result of our project. Additionally, it does not pose a threat to public health, as no toxins, chemicals, or any dangerous substances are being produced. Furthermore, our project does not serve as a stepping stone for potential bioweapons.
Environmental Safety
In any type of genetic engineering, there is always the hidden danger of horizontal gene transfer between the synthetic organism and other microbes co-existing with the synthetic organism. Thus, the possibility of horizontal gene transfer must be taken into consideration if and when the synthetic E. Coli enters the real world.
If our strain of E. Coli ever gets leaked outside of the lab, there would be no effect on the surrounding environment because it would not grow well in soil in the first place. In order to grow it effectively in lab, we had to grow it on nutrient-rich broth or agar plates and incubate them in a warm room overnight.
If our E. Coli were able to survive in the outdoors, no effect will be made on the ecosystem. Microbes that receive our E. Coli’s plasmids will not gain any selective advantage over other microbes, since our plasmids were designed to create a visual pattern. They may, however, receive some slight antibiotic resistance due to the fact that our plasmids have antibiotic resistance for use as selective markers in the lab, but this is not significant enough to be considered a selective advantage.
Should our E. Coli pick up pathogenic genes from other microbes, our E. Coli would be just as harmful as if it was a normal E. Coli. We did not give our E. Coli any selective advantages, other than the few antibiotic resistance genes as selective markers for use in the lab.
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