Team:Cambridge/PhosphoreumOligos

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Phosphoreum Oligos

ribA Reverse Primer: Sequence: tactagtagcggccgctgcagAAATGAACGTTAGTTCCAAC Structure: one hairpin in suffix/coding region Tm: 56.27 deg dG: 0.48 kcal/mol

luxE Reverse Primer: Sequence: tactagtagcggccgctgcagTATGATCTATTTTAAACCTGATGA Structure: one hairpin in suffix Tm: 56.59 deg dG: 0.57 kcal/mol

Pbad Reverse C-end: Sequence: tctttatcatacaatctccttaGCTAGCCCAAAAAAAC Structure: one small hairpin in gene Tm: 53.68 deg dG: 2.30 kcal/mol

Lux C forward primer: Sequence: AGGAGATTGTATGATAAAGAAAAT Tm: 56.26 dG: 1.98

lux A(XbaI)

forward TACTGGACActagaGCATCATT Tm=58.46 dG=1.53

reverse AATGATGCtctagTGTCCAGTA Tm=58.46 dG=2.53

lux E(SpeI)

forward GTAGTGGTacCagtGGTGTAAA Tm=58.54 dG=1.41

reverse TTTACACCactGgtACCACTAC Tm=58.54 dG=1.25

lux G(XbaI) forward TGCCTGTCctagaAAACTTT Tm=58.56 dG=1.77

reverse AAAGTTTtctagGACAGGCA Tm=58.56 dG=1.33

ribB (EcoRI i)

forward GAATATGGTgaGttcAATATGATC Tm=58.39 dG=1.91

reverse GATCATATTgaaCtcACCATATTC Tm=58.39 dG=1.91

ribB (EcoRI ii)

forward TGAGCCgaaCtcTCAAAC Tm=59.64 dG=1.26

reverse GTTTGAgaGttcGGCTCA Tm=59.64 dG=1.16