Team:Cambridge/OligoOrderVibrio13.08.10

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BioBrick Prefix:

If following part is coding or contains ATG:

gaattcgcggccgcttctag

Otherwise:

gaattcgcggccgcttctagag

BioBrick Suffix:

tactagtagcggccgctgcag

Pbad Forward Prefix Primer:

Sequence: gaattcgcggccgcttctagagTTATGACAACTTGACGG

Structure: one hairpin in gene

Tm: 52.95 deg

dG: 1.00 kcal/mol

Pbad Reverse Oend

Sequence: tagtcataccaacctcccttgcgGCTAGCCCAAAAAAAC

Structure: one hairpin in gene

Tm: 53.68 deg

dG: 1.11 kcal/mol

luxR forward primer

Sequence: ATGAAAAACATAAATGCCG

Tm: 58.06 deg

dG: 1.59 kcal/mol

luxR reverse Iend

Sequence: atgatcatcgccggcgacgtcCTCTAGTAGTGATCTACACTAGCACTATC

Tm: 58.54 deg

dG: 0.34 kcal/mol

luxI forward primer

Sequence: AAGGGAGGTTGGTATGACTATA

Tm: 58.52 deg

dG: 2.41 kcal/mol

luxG reverse suffix primer (finishing before the final terminator)

Sequence: atgatcatcgccggcgacgtcTTTATATTCTCTTTATACGTATGCAA

Tm: 57.36 deg

dG: -0.34 kcal/mol

luxO forward primer

Sequence:AGGGAAAATAATGAATAAATGTATT

Tm: 57.29 deg

dG: 2.13 kcal/mol

luxO reverse suffix primer (finishing at RBS of luxG)

Sequence: atgatcatcgccggcgacgtcAACTTAATCCTTGATATTCTTTTGTAT

Tm: 57.96 deg

dG: -0.34 kcal/mol

Pbad Reverse Rend

Sequence: ttcatctagtatttctcctctttGCTAGCCCAAAAAAAC

Structure: one hairpin in gene

Tm: 53.68 deg

dG: 1.83 kcal/mol

luxO forward prefix primer

Sequence: gaattcgcggccgcttctagagAGGGAAAATAATGAATAAATGTAT

Tm: 56.07 deg

dG: 0.76 kcal/mol