Team:KAIST-Korea/Project/References

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Data sources

During our project, we needed many data. We used data of protein-protein interactions for signal transduction pathway deciding, protein sequence and domain description for modified FGFR design, gene sequence for modified FGFR sequence synthesis, and structures or sequences of antibodies and FGF binding domain of FGFR for structural alignment. These data were acquired from Uniprot, PID, NCBI and RCSB PDB.

Uniprot

UniProt.png

Uniprot provides sequences of many proteins and domain description of well-researched proteins. Key advantage of using Uniprot is domain description: to design engineered protein for detecting Mycobacterium without knowledge of function of each part of protein. Tuberculosis antigen MPT51 is impossible one. Uniprot provided the information of location of FGF binding domain of FGFR, which is replaced by our single-chain antibody 16A1. Uniprot also provided sequences of some antibodies to make single chain antibody sequences.

  • Site URL: http://www.uniprot.org/

PID

PID.png

PID (Pathway Interaction Database) provides the interaction networks between protein and protein or protein and DNA through certain signal transduction pathway. This Protein-protein interaction (PPI) and protein-DNA interaction (PDI) data helped us to port Human signal transduction pathway which is activated by FGF to fission yeast. Without knowledge of PPI and PDI through FGF signal pathway, we must undergo many trials and errors of adding and removing of proteins and promoters to form working signal transduction pathway. With the data from PID, we decided to port the FGF->FGFR1->STAT1->GAS pathway from human to fission yeast.

  • Site URL: http://pid.nci.nih.gov/

NCBI

NCBI.png

NCBI provides biologists with many data. We used protein sequences and DNA sequences from NCBI nucleotide database and protein database. DNA sequence is very important to us because gene sequence of original protein is required to synthesize novel engineered protein. Even we don't synthesize gene, we should know the sequence of gene because BioBrick requires not only nucleotide material, but also its sequence information. NCBI also provided us with sequences of some antibodies to make single chain antibody sequence, and many Journals through PubMed service.

  • Site URL: http://ncbi.nlm.nih.gov/

RCSB PDB

RCSB-PDB.png

RCSB PDB (Protein Data Bank) provides the data of structure of protein or other biomolecules. Key feature of data from PDB is structure. NCBI or Uniprot provide the sequence of proteins, but it does not show us their 3D-structures. With the structural similarity between FGF binding domain of FGFR and single chain antibody 16A1, we can ensure that the replacement of FGF binding domain with 16A1 to detect MPT51 is appropriate.

  • Site URL: http://www.pdb.org/

Tools

During our project, we processed much bio-information. It is not easy to process that massive information manually, so we used many bioinformatics tools for our projects. We marked restriction sites to select proper restriction enzyme, searched the nucleotide which codes query peptide sequence to find the coding region of certain genes, virtual-translated given nucleotide sequence to check if our sequence codes expected protein, and predicted and aligned structure of single chain antibodies with FGF binding domain of FGFR to check single chain antibodies are structurally similar to confirm that replacement of FGF binding domain with single chain antibodies is appropriate. We used BioEdit to mark restriction site, BLAST to find the coding region of certain genes and to compare similar proteins, Transeq for virtual-translation, Modeller for structural prediction of single chain antibody, and Matt for structural alignment between single chain antibodies and FGF binding domain of FGFR.

BioEdit

BioEdit.png

BioEdit is the program for displaying biological sequences. It displays different amino acids or nucleotides in different colors to allow its users to check the change between sequences. It has many simple but useful functions. We used BioEdit for marking the restriction sites on given sequence to select proper restriction enzyme, which does not restrict coding region of gene. Other functions of BioEdit like phylogeny making or front-end of ClustalW are not used in our project, but they are also useful.

  • Supporting Platform: Windows
  • License: Freeware
  • Download: http://www.mbio.ncsu.edu/BioEdit/bioedit.html

BLAST

BLAST.png

BLAST is the alignment search tools for protein or nucleotide sequences. There are five modes of BLAST; blastn (nucleotide to nucleotide), blastp (Protein to Protein), blastx (nucleotide to protein), tblastn (protein to nucleotide), and tblastx (translated nucleotide to translated nucleotide). We used tblastn to find the location of coding region of given protein and blastn to find the differences between transcription variants of same genes.

  • Web service: http://blast.ncbi.nlm.nih.gov/Blast.cgi
  • Supporting Platform: Solaris, Linux, Windows, and Mac OS X
  • License: Public Domain
  • Download: http://blast.ncbi.nlm.nih.gov/Blast.cgi?CMD=Web&PAGE_TYPE=BlastDocs&DOC_TYPE=Download
    • For PowerPC G4/G5 processors and Mac OS X, optimized version of standard BLAST is also available [http://developer.apple.com/opensource/tools/blast.html here]

Transeq

Emboss.png

It is not difficult to make protein sequence from nucleotide sequence. With codon table, we can make protein sequence manually without any special ability. To translate long nucleotide sequence, however, is not easy. Therefore, we used Transeq to virtually translate given nucleotides sequence. Transeq also do virtual-translation with shifted reading frame or non-transitional translation code tables like mitochondrial translation code table.

  • Web service: http://www.ebi.ac.uk/emboss/transeq/
  • Supporting Platform: Basically Linux. some porting projects are available.
  • License: GNU General Public License as EMBOSS package
  • Download: http://emboss.sourceforge.net/download/
    • For windows, [http://emboss.sourceforge.net/download/cygwin.html CygWin notes] may be helpful
    • For Macintosh, [http://emboss.sourceforge.net/download/macosx.html Mac OS X notes] may be helpful

Modeller

Modeller.png

Modeller is the program to predict the structure of protein with given peptide sequence based on the homology model. When given query sequence, Modeller searches for the similar sequence from database sequence whose structure is known. It assumes that similar sequences have similar structures and predicts the structure of query protein as the combination of known structure with similar sequence. This method is very useful for prediction of single chain antibodies because the structures of many original antibodies are known.

  • Supporting Platform: Unix, Linux, Windows, and Mac
  • License: Free fore non-profit academic institutions
  • Download: http://www.salilab.org/modeller/download_installation.html

Matt

Matt.png

It is also possible to compare the structures between FGF binding domain of FGFR and single chain antibodies by using Modeller manually, but it is not quantitative and estimated by the rule of thumb. So, the result is not useful for further analysis. We used Matt to compare the structures of FGF binding domain of FGFR and single chain antibodies. Matt uses algorithms to maximize shared structure with small translation and rotations. Matt provides quantitative results to enable estimation of similarity and aligned structures of proteins to visualize the alignment.

  • Supporting Platform: Unix, Linux, and Windows
  • License: GNU public license
    • Commercial Matt license is available through the MIT and Tufts offices of Technology Transfer for a non-GPL software package.
  • Download: http://groups.csail.mit.edu/cb/matt/

PyMol

PyMol.png

Structure of complex of protein and other biomolecules are often saved as format of “*.PDB”. Visualization and analysis of that structure and sequence are also important to design novel engineered proteins. PyMol is used for such purposes. We used PyMol for two processes. First of all, it is used to confirm iG-like regions of FGFR are really FGF binding domain. We downloaded the FGF binding domain of FGFR from RCSB PDB and checked the sequence binds to the FGF is really marked as iG-like regions. (Interleukin receptors have iG-like regions, but they do not bind to their signal molecules.) Other process using PyMol is to visualize the structural alignment result made by Matt. PyMol saves the image of biomolecules in png format.

  • Supporting Platform: Unix, Linux, Windows and Mac OS X
  • License: Free for Older builds, registering is required for recent version
  • Download: [http://pymol.org/rel/099/ free older builds] and [http://pymol.org/rel/ registering page]

Other Media Tools

  • Wiki decoration with images : Adobe Photoshop and MS paint
  • Wiki decoration with flash movie : Adobe Plash
  • Movie production for presentation : Vegas Movie player

References

Fig 1. Estimated TB incidence rates, 2008 WHO Library Cataloguing-in-Publication Data,
Global tuberculosis control: a short update to the 2009 report
Fig 2. People suffering from malaria and TB
Mother nature network, article “New form of malaria threatens Thai-Cambodia border”
Fig 3. Researching disease
Fondation Merieux Research Programs
http://www.fondation-merieux.org/-research-programmes.html
Fig 4. Antibody
Cytomx http://cytomx.com/technologies.html
Fig 5. MPT51
RCSB Protein Database, "The crystal structure of Mycobacterium tuberculosis MPT51"
Fig 6. E.coli Statistics
http://redpoll.pharmacy.ualberta.ca/CCDB/cgi-bin/STAT_NEW.cgi
Fig 7. Yeast homologous recombinant
Bioneer http://pombe.bioneer.co.kr/technic_infomation/construction.jsp
Fig 8. CRYSTAL STRUCTURE OF A TERNARY FGF2-FGFR1-HEPARIN COMPLEX
RCSB Protein Database, "CRYSTAL STRUCTURE OF A TERNARY FGF2-FGFR1-HEPARIN COMPLEX"
Fig 9. FGF signaling pathway
Nature Pathway Interation Database, "FGF signaling pathway"
http://pid.nci.nih.gov/search/pathway_landing.shtml?pathway_id=fgf_pathway&source=NCI-Nature%20curated&what=graphic&jpg=on&ppage=1
Fig 10. Unneccesary Syndecan-2 Function
http://www.kaomp.org/new/board_view.htm?table_name=photo_0&currPage=6&aq_id=92&aq_type=&aq_value
Fig 11. Structural bases of unphosphorylated STAT1 association and receptor binding
RCSB Protein Database, "Structural bases of unphosphorylated STAT1 association and receptor binding"
Fig 12. Roberto, Proteína fluorescente verde – história e perspectivas, Química de Produtos Naturais (2009)
Fig 13. KAIST success!
http://imperfectaction.com/blog/2009/03/04/entrepreneurship/definition-of-success/


[1] International Union of Pure and Applied Chemistry. "biosensor". Compendium of Chemical Terminology :: Internet edition.
[2] Garrett, R.H., and Grisham, C.M. Biochemistry, 2nd Edition (2002), pg. 32
[3] RCSB PDB(protein database), http://www.rcsb.org/
[4] Walker K, Skelton H, Smith K. (2002). accessdate=2009-11-28 "Cutaneous lesions showing giant yeast forms of Blastomyces dermatitidis". Journal of Cutaneous Pathology 29 (10): 616–18. doi:10.1034/j.1600-0560.2002.291009.x
[5] LUCI´A CITORES,1* LING BAI,2 VIGDIS SØRENSEN,2 AND SJUR OLSNES2, Fibroblast Growth Factor Receptor-Induced Phosphorylation of STAT1 at the Golgi Apparatus Without Translocation to the Nucleus, Cellular Physiology (2007)
[6] Ronit A loni-Grinstein, Andrew Seddon, 1 and A vner Yayon*, Reconstitution of Fibroblast Growth Factor Receptor Interactions in the Yeast Two Hybrid System, MOLECULRR BIOTECHNOLOGY (1999)
[7] Fariba Barahmand-Pour, Andreas Meinke, Bernd Groner‡, and Thomas Decker§, Jak2-Stat5 Interactions Analyzed in Yeast*, The American Society for Biochemistry and Molecular Biology (1998)
[8] Hong Xu, Kyung W. Lee, and Mitchell Goldfarb‡i, Novel Recognition Motif on Fibroblast Growth Factor Receptor Mediates Direct Association and Activation of SNT Adapter Proteins*, The American Society for Biochemistry and Molecular Biology (1998)
[9] M. Menke, B. Berger, L. Cowen, "Matt: Local Flexibility Aids Protein Multiple Structure Alignment", PLoS Computational Biology (2007)