Team:Stanford/Notebook/Lab Work/Week 7
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8/9 Monday
Laura's Notebook
- helped Alex NanoDrop some of his samples (concentrations also written on tops of tubes)
260/280 | 260/230 | ng/uL | |
sGFP mDNA | 1.71 | 1.01 | 320.3 |
sGFP mDNA s | 1.91 | 1.41 | 220.7 |
F2620 | 1.10 | 0.87 | 45.0 |
F2620 s | 1.52 | 0.88 | 53.1 |
I5 | 1.90 | 1.06 | 165.8 |
I5s | 1.98 | 1.22 | 113.9 |
I4 | 1.73 | 0.95 | 384.2 |
I0500 | 1.86 | 0.89 | 67.7 |
C1 | 1.77 | 0.80 | 83.0 |
C2 | 1.83 | 0.83 | 86.6 |
C3 | 1.95 | 1.09 | 75.7 |
8/10 Tuesday
Laura's Notebook
set up colony PCR for single colony from pLUX-sRNA1C ligation/transformation:
- 9 uL supermix, 0.5 uL of each primer (VF, VR)
- cycling program as before: COLPCR72
3pm meeting with Drew, Rayka about device names, parameters