Team:Groningen/Notebook/Peter

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Bascillus Subtilus, easy info:

http://www.mobitec.de/int/products/bio/04_vector_sys/index.php?bac_sub.html
http://subtiwiki.uni-goettingen.de/wiki/index.php/SubtiPathways
http://subtiwiki.uni-goettingen.de/wiki/index.php/Main_Page

09/08

Cell wall isolation according to protocol: Cell disruption, samples are frozen.

Culture Bascillus over night, all four strains: Rok, DegU, SigF, Sp0A

10/08

Cell wall isolation according to protocol: Cell disruption again, continue according to protocol

Dry-freeze over night

Culture Bascillus over a extra night, all four strains: Rok, DegU, SigF, Sp0A

11/08

Chaplin isolation:

4x approximately 15 mg dry cell wall (180 ul TFA), 4x approximately 10 mg dry cell wall (100 ul TFA), 8x approximately 5 mg dry cell wall (100 ul TFA),

Cultured bascillus strains are taken out of the stove, medium will be used for degredation experiment.

DEGREDATION EXPERIMENT:

3x appr. 5 mg samples + ROK, DegU, SigF medium (+ 1 control normal medium) 3x appr. 5 mg samples + ROK, DegU, SigF lysed medium (+ 1 control lysis normal medium) 3x appr. 15 mg samples + ROK, DegU, SigF medium (+ 1 control normal medium) 3x appr. 10 mg samples + ROK, DegU, SigF medium (+ 1control normal medium)