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From 2010.igem.org

Contents 1 Andreas 1.1 Team logotype 2 Hassan 2.1 MITF-PAX3 3 Mimmi 3.1 MITF 3.1.1 - Gel 3.1.2 - amplifying and moving

Andreas

No lab work.

Team logotype

Worked on the team logotype most of the day. I have made a few different versions to be voted for.

Hassan

MITF-PAX3

"...Pax3 simultaneously functions to initiate a melanogenic cascade while acting downstream to prevent terminal differentiation. Pax3 activates expression of Mitf, a transcription factor critical for melanogenesis while at the same time it competes with Mitf for occupancy of an enhancer required for expression of dopachrome tautomerase, an enzyme that functions in melanin synthesis." [http://www.ncbi.nlm.nih.gov/pubmed/15729346]

Mimmi

MITF

- Gel

• No products!

--> Trying a different polymerase

- amplifying and moving

Mix	(µl)	X18		primers		conditions
sH2O	22	396		MITF_F		time	°C
F primer	1	18		MITF_R		2m	94
R primer	1	18				30s	94	)
DNA	1	18				30s	45, 51, 55	> 5 cycles
tot	25	450				3m	72	)
						30s	94	\
						3m	72	/ 25 cycles
						10m	72	rowspan="2"
						oo	10