Tsinghua University

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Our Project

Tsinghua iGEM 2010 Project is focused on developing a new Antibody Production Method using Synthetic Biology. The Aim is to Simulate Antibody Selection and Production Technology with Bacteria.

Traditional method of antibody production such as Hybridoma Technology is expensive and time consuming. In Hybridoma Technology, generation of B-Cell library is accomplished inside experimental animal, mouse for example; using Flow Cytometry to select a specific kind of antibody; then hybridoma would be prepared to harvest antibody.

In this project, we expect to achieve all these processes cheap and efficiently in E.coli Cell. The whole project can be divided into two modules:

MODULE I: Generation of Antibody Library

MODULE II: Selection of Specific Antibody

===中文版===

2010年清华大学iGEM项目的重点是开发新的生产方法使用抗体合成生物学。其目的是模拟抗体的选择和生产工艺细菌。 传统方法生产的抗体，比如杂交瘤细胞等技术，是昂贵和费时。杂交瘤技术，对B-细胞库构建是通过实验动物，比如老鼠;使用流式细胞仪来选择一个特异性抗体，然后用杂交瘤细胞收获抗体。 在这个项目中，我们期待在大肠杆菌细胞中用一种廉价高效的方法实现所有这些过程。整个项目可分为两个模块：

模块 I: 抗体库的构建

模块 II: 特异性抗体的筛选