Team:ETHZ Basel/Biology/Status

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Revision as of 15:03, 6 August 2010 by Luzi (Talk | contribs)

generation of subparts

subparts
Protein primers PCR product library of transformants single colonies storage vector insert
che proteins CheY-A recieved purified on stock on agar plate not available purified on stock on stock
CheY-C recieved purified in stock on agar plate not available purified on stock on stock
CheB-A recieved purified in stock on agar plate 3 on agar plate purified on stock on stock
CheB-C recieved purified in stock on agar plate 3 on agar plate purified on stock on stock
CheR-A recieved purified in stock on agar plate 3 on agar plate digest has to be redone
CheR-C recieved purified in stock on agar plate 3 on agar plate digest has to be redone
localizers TrigF-A recieved purified in stock on agar plate 3 on agar plate purified on stock
MreB-C recieved purified in stock in process
tetR-A recieved purified in stock on agar plate 3 on agar plate purified on stock on stock
couplers PIF3-A recieved purified in stock on agar plate 3 on agar plate purified on stock
PIF3-C recieved purified in stock on agar plate 3 on agar plate purified on stock
PhyB-C recieved purified in stock on agar plate 3 on agar plate purified on stock
reporters cyFP-A recieved purified in stock on agar plate 3 on agar plate purified on stock on stock
cyFP-C recieved purified in stock on agar plate 3 on agar plate purified on stock


generation of biobricks

testing of che-fusions

We're currently trying to establish an assay to test for the chemotactic behavour of E. coli. A first try with a "syringe test" failed. We'll redo the test and are looking for other possibilites. Maybe a swarm test?


testing of localicer-fusions

We are planning to use a fluorescence microscope in order to visualize the distribution of localizer-cyFP fusions within a single E. coli.

testing of couplers

We're planning to use the setup as for the testing of the localizers. In this case we're going to use the following biobricks:

  • localizer-PhyB and PIF3-cyFP or
  • localizer-PIF3 and PhyB-cyFP

Images should look as in testing of localizers
Preliminary clarificaiton of the suitability of the approach are to be done.

testing of biobricks

bringing E. lemming alive

timetable

experimental plan