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JUNE
Finally Bio-Lab activity began! Select the day you are interested to find out all the details.
| DATE | ACTIVITY |
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| June, 3rd | 9° Meeting - Discussion of final and technical details about The Project. Planning of Bio-Lab activity. |
| WEEK 2 |
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| June, 7th | <partinfo>BBa_E2050</partinfo>, <partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo> were resuspended from Spring 2010 DNA distribution. <partinfo>BBa_P1004</partinfo> was resuspended from Spring 2009 DNA distribution. All BioBricks were transformed in E. coli DH5alpha.
Liquid LB+Amp and LB+Amp agar plates were prepared according with "protocols". |
| June, 8th | Inoculum from plates in liquid Lb and glycerol stocks preparation. Falcon were re-filled for tomorrow mini-prep.
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| June, 9th | MiniPrep of <partinfo>BBa_E2050</partinfo>, <partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo>. Digestion, gel run/cut and ligation of:
- I0: <partinfo>BBa_K125500</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X)
- I1: <partinfo>BBa_E2050</partinfo> (E-S)+<partinfo>BBa_K165018</partinfo> (E-X)
- I2: <partinfo>BBa_P1004</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X)
- I3: <partinfo>BBa_K081008</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X)
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| June, 10th | Transformation of ligations I0, I1, I2, I3 in home made E. coli DH5-alpha competent cells. Transformants were plated on LB+Amp agar plates.
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| June, 11th | Inoculum from plates in liquid LB and glycerol stocks preparation for I0, I1, I2 and I3 in duplicate (2 colonies peaked from each plate).
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| June, 12th | iGEM EU workshop.
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| June, 13th | iGEM EU workshop.
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| WEEK 3 |
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| June, 14th | 6° Bio-Lab - Lorenzo and Nicolò.
Inoculum from glycerol stock of I0-1, I0-2, I1-1, i1-2, I2-1, I3-1 and I3-2 fro screening. |
| June, 15th | 7° Bio-Lab - Lorenzo and Manuel L., Sara and Alessandro. MiniPrep, gel run/cut and ligation of
- I4: <partinfo>BBa_K165037</partinfo> (S-P) + I1 (X-P)
- I5: I2 (E-S) + <partinfo>BBa_J61001</partinfo> (E-X)
- I6: I3 (E-S) + <partinfo>BBa_T9002</partinfo> (E-X)
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| June, 16th | 8° Bio-Lab - Susanna Z., Sara and Nicolò. Tranformation of I4 (DH5-alpha), I5 (TOP10) and I6 (DH5-alpha).
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| June, 17th | 9° Bio-Lab - Susanna Z., Nicolò and Riccardo. Colonies were peaked from each plate, I4-1, I4-2, I5-1, I5-2, I6-1, I6-2 and I6-3 were grown in 1ml liquid culture and glycerol stocked (not I5-2, because no growth was observed in LB+Cm and not for I6-1, not grown). Cultures refilled with 5ml LB+antibiotic for tomorrow MiniPrep and screening.
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| June, 18th | 10° Bio-Lab - Susanna Z., Susanna S. and Federica. MiniPrep for I4-1, I4-2, I5-1, I6-2 and I6-3. Screening gel (all the clones are ok!) :)
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| WEEK 4 |
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| June, 21st | 11° Bio-Lab - Nicolò, Manuel Z., Susanna Z.
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| June, 22nd | 12° Bio-Lab - Susanna Z., Lorenzo, Sara, Nicolò.
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| June, 23rd | 13° Bio-Lab - Sara, Susanna Z., Nicolò.
10° Meeting - Updating about Bio-Lab activity. |
| June, 24th | 14° Bio-Lab - Sara, Susanna Z., Alessandro.
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| June, 25th | 15° Bio-Lab - Susanna Z., Chiara, Riccardo.
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| June, 28th | 16° Bio-Lab - Susanna Z., Nicolò, Federica, Giacomo.
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| June, 29th | 17° Bio-Lab - Susanna Z., Nicolò, Riccardo,Giacomo, Lorenzo.
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| June, 30th | 18° Bio-Lab - Susanna Z.
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