Week of 7/5
From 2010.igem.org
Contents |
July 5th
- The university was closed due to the holiday on July 4th.
July 6th
- Back to work!
- Tomorrow:
- Retransform: o All lux promoters from kit plates.
- Grow for tomorrow from stock:
- R0061 - J24679 - R1062 - K112022 - K091107 - K091146 - K145150
- Cleaned cuvettes for transformations.
- Worked on Human Practices survey.
July 7th
- Flasks minipreped:
- J24679 - R1062 - K112022 - K091107 - K091146 - K145150
- Sent to sequencing:
1) P0451 2) K112022 3) J24679 4) K091146 5) K145150 6) R0010 7) R1062 8) K091107
- Digests:
- J24679 (ES+phosphatase) - P0451 (XP+phosphatase) - K274100(ES+phosphatase) - S01414 (ES+phosphatase) - K274100(XP+phosphatase) - B1002 (EX) - B1006 (EX) - R0010 (SP) - B1002 (EX) - B1006 (EX) - J23100 (SP)
- Gel order:
- Ladder - B1006 - J23100 - R0010
All three bands appeared around the base pairs that we wanted then at so the 3 bands were cut out and then gel purified.
- Ordered 3 primers.
- Ligations:
Insert Vector
1) J24679 B1006 2) P0451 R0010 3) K274100 B1006 4) S01414 B1006 5) K274100 J23100
July 8th
- Colony PCR:
1) P0451+R0010 A-D 2) K274100+B1006 A-D 3) K274100+J23100 A-D 4) J24679+B1006 A-D
- Digest:
- J24679 (ES+phosphatase) - K274100(ES) - R0010 (SP) - B1002 (EX) - K112022 (XP) - K112808 (XP)
- Gel order:
Upper-digests - Ladder - K274100 - K112808 - K1120222 - B1002 Lower-PCR - Ladder - 1 A-F - 4 A-E
- We forgot to put the Taq polymerase and so as you can see, nothing showed up in the gel.