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From 2010.igem.org

Media Recipes

LB media

• 10 g LB broth
• 500 mL DI water

autoclave to sterilize

LB media with agar

• 10 g LB broth
• 7.5 g agar
• 500 mL DI water

autoclave to sterilize

add antibiotics after autoclaving when warm to touch (about 50°C)

pour plates when warm to the touch (about 50°C)

0.1 M CaCl2 solution

• 0.735 g of CaCl2*2 H20
• 50 mL of DI water

filter sterilize

1.0 M CaCl2 solution

• 7.35 g of CaCl2*2 H20
• 50 mL of DI water

filter sterilize

1.0 M MgSO4

• 12.323 g of MgSO4*7 H20
• 50 mL of DI water

filter sterilize

Ampicillin Antibiotic Stock Solution (100 mg/mL)

• 1 g of ampicillin
• 5 mL of DI water
• 5 mL of ethanol

filter sterilize and make 1 mL aliquotes to store in the -20°C freezer

Kanamycin Antibiotic Stock Solution (50 mg/mL)

• 250 mg of kanamycin
• 5 mL of DI water

filter sterilize and make 1 mL aliquots to store in the -20°C freezer

Tetracycline Antibiotic Stock Solution (50 mg/mL)

• 250 mg of tetracycline
• 2.5 mL of DI water
• 2.5 mL of ethanol

filter sterilize and make 1 mL aliquots to store in the -20°C freezer

IPTG stock solution (1 M)

• 0.25 g of IPTG
• 1049 uL of DI water

filter sterilize with .22 um filter

Cyclohexanecarboxylic acid

Cyclohexanecarboxylic acid is volatile should only be worked with in a hood. For accurate measurement use covered scale with sliding doors into the hood. Stored in the corrosive cabinet

• 0.075 g cyclohexanecarboxylic acid
• 50 mL of DI water

filter sterilize