Team:Cambridge/Notebook/Week3
From 2010.igem.org
![E. glowli](https://static.igem.org/mediawiki/2010/d/d4/CamBillHeaderSq.png)
Week 3
Monday
Afternoon
- Theo created a plan for an LRE Biobrick, and submitted it to DNA 2.0 for a quote.
- Anja, Hannah and Emily worked on text for Sterilin
- Peter and Will created primer sequences for first experiment
Tuesday
- Duncan provided several E. coli strains. We plated out TOP10 to create colonies from which to create competent cells for transformation.
- We organised all the stock in the lab
- We met with Dr. Summers to discuss quiescence. He was very encouraging but have yet to settle IP concerns. He also suggested considering mopping up Rcd from a leaky inducible promoter with antisense RNA.
Wednesday
Inoculated a broth with Top 10. Making competent cells tomorrow.
Thursday
To do: prepare competent cells
Friday
- Preparing order for sythesis
- Theo aligned 300 luciferase-sequences to visualise consensus
- Peter and Theo discovered DINAmelt, downloaded UNA Fold to model Rcd dynamics
- Anja, Emily and Bill prepared chemically competent cells!
- By spending an hour in a cold room. It was cold but worth it. We got to drink hot chocolate.
Saturday
- Fernan tested our competent cells for us and they worked!
Sunday
- Anja, Bill, Emily and Theo discussed a final template for the wiki - it is looking pretty.