"

From 2010.igem.org

We're a team composed of mainly undergraduates from the Bioengineering course, but this year we have people from Applied Chemistry, Applied Physics and Medicine in our team too! Together we're gonna do something GREAT!!
	Your team picture
	iGEM Osaka 2010

Home	Team	Project	Submitted Parts	Modeling	Notebook	Safety

Contents 1 Notebook 1.1 July 29 (Thu) 1.2 July 31 (Thu) 1.3 August 2 (Mon)

Notebook

August M T W T F S S             [http://2010.igem.org/wiki/index.php?title=August/1_August_2010&action=edit 1] [http://2010.igem.org/August/2_August_2010 2] [http://2010.igem.org/wiki/index.php?title=August/3_August_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=August/4_August_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=August/5_August_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=August/6_August_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=August/7_August_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=August/8_August_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=August/9_August_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=August/10_August_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=August/11_August_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=August/12_August_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=August/13_August_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=August/14_August_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=August/15_August_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=August/16_August_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=August/17_August_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=August/18_August_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=August/19_August_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=August/20_August_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=August/21_August_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=August/22_August_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=August/23_August_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=August/24_August_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=August/25_August_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=August/26_August_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=August/27_August_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=August/28_August_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=August/29_August_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=August/30_August_2010&action=edit 30] [http://2010.igem.org/wiki/index.php?title=August/31_August_2010&action=edit 31]

September M T W T F S S     [http://2010.igem.org/wiki/index.php?title=September/1_September_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=September/2_September_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=September/3_September_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=September/4_September_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=September/5_September_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=September/6_September_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=September/7_September_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=September/8_September_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=September/9_September_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=September/10_September_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=September/11_September_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=September/12_September_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=September/13_September_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=September/14_September_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=September/15_September_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=September/16_September_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=September/17_September_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=September/18_September_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=September/19_September_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=September/20_September_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=September/21_September_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=September/22_September_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=September/23_September_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=September/24_September_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=September/25_September_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=September/26_September_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=September/27_September_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=September/28_September_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=September/29_September_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=September/30_September_2010&action=edit 30]

October M T W T F S S         [http://2010.igem.org/wiki/index.php?title=October/1_October_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=October/2_October_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=October/3_October_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=October/4_October_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=October/5_October_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=October/6_October_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=October/7_October_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=October/8_October_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=October/9_October_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=October/10_October_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=October/11_October_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=October/12_October_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=October/13_October_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=October/14_October_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=October/15_October_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=October/16_October_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=October/17_October_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=October/18_October_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=October/19_October_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=October/20_October_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=October/21_October_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=October/22_October_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=October/23_October_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=October/24_October_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=October/25_October_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=October/26_October_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=October/27_October_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=October/28_October_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=October/29_October_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=October/30_October_2010&action=edit 30] [http://2010.igem.org/wiki/index.php?title=October/31_October_2010&action=edit 31]

November M T W T F S S [http://2010.igem.org/wiki/index.php?title=November/1_November_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=November/2_November_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=November/3_November_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=November/4_November_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=November/5_November_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=November/6_November_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=November/7_November_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=November/8_November_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=November/9_November_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=November/10_November_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=November/11_November_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=November/12_November_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=November/13_November_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=November/14_November_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=November/15_November_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=November/16_November_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=November/17_November_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=November/18_November_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=November/19_November_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=November/20_November_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=November/21_November_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=November/22_November_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=November/23_November_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=November/24_November_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=November/25_November_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=November/26_November_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=November/27_November_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=November/28_November_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=November/29_November_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=November/30_November_2010&action=edit 30]

Note: For detailed lab notes see individual pages in calendar above.

July 29 (Thu)

Attendance: Torigata, Takino, Teoh, Yasumoto, Kakuda, Saka, Tamura

1. Safety lecture for junior members.
2. Preparation of LB agar plates (26 Amp, 25 Kan, 25 Cam).

July 31 (Thu)

Attendance: Miyatake, Hirayama, Torigata, Teoh, Tadashi, Yasumoto, Kakuda, Saka

1. Meeting
   1. Summer project schedule
   2. List of genes to clone

August 2 (Mon)

Attendance: Torigata, Takino, Teoh, Tadashi, Yasumoto, Saka

1. Culture medium preparation
   1. LB agar plates (49 antibiotic-less plates)
   2. LB liquid medium (500 ml)
2. Competent cells preparation - Nojima Method
   1. SOB medium (MgCl2 not yet added) -> stored at 4˚C
   2. TB buffer -> stored at 4˚C
   3. Single-colony streaking of E. coli (strain DH5α) on 2 LB agar plates -> 37˚C incubation o/n

Tomorrow we shall prepare the remainder of the reagents and inoculate SOB with o/n-incubated E. coli.