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From 2010.igem.org

Contents 1 Mimmi 1.1 LMWP C and N 1.2 over-expression in pEX - remaking the comassie-gel 2 Nina 3 Sequencing CPP transportan 10 version C 4 Inoculation of IgG protease

Mimmi

LMWP C and N

Plasmid prep and glycerol stock

The cells were cultured to 0.6 OD, 1.6 ml were used to the glycerol stock and 10 ml were used to extract the plasmid

DNA concentrations

Sample	DNA (ng/μl)
LMWP N	60
LMWP C	80

over-expression in pEX - remaking the comassie-gel

MIX:

1. 4ml LB
2. 40µl glycose (1%)
3. 8µl Amp (0,5%)

Add a pipette tip of BL21 pEX.BBa_J18932 from glycerol stock and grow ON

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Nina

Sequencing CPP transportan 10 version C

I send two samples of CPP transportan 10 version C with colony number 9 and 40 for sequencing.

• 15 ul vector

• 1.5 ul 10uM VR2 primer

9: ASB003N X99

40: ASB003N X98

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Inoculation of IgG protease

I placed the inoculation samples in the refrigerator for mini prep the following day when I know which sample is suitable for miniprep.