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From 2010.igem.org

July 30, 2010

Team Meeting Today!

After eating our pizza, we started discussing the separate components of the biosensor.

First, we discussed the lab work results over the past couple of days with low yields and differing colors. We figured out it was because of the different E.coli strains of DH5α and MG155 used. Instead of using the MG155 used in the last step, we were supposed to use the high efficiency DH5α strain in the transformations to obtain DNA strain.

Plan for Monday: Retransform all of the plasmids.

AROMATICS/NITRATES

Next we discussed the aromatic compounds/nitrate sensor components. We went through the plan that Kristina sent including the possible logic gates. We went through the plasmids of Pr + XylR and the PyeaR promoters including the backbone of PsB1A2. In addition, we discussed the logic gates of the aromatics and the nitrates.

ALKANES

Sameer presented his plan on the alkane hydroxylase with luciferase plan along with his idea about the oxidation of cylcloalkanes instead of the alkanes because of the large size of the allkane parts (35 kbp).

UTD LOGO

In addition, as a supplement to our main project on petroleum sensing, we are conducting a mini-project on producing a UTD logo with colored bacteria based on a photo-sensitive system. LP brought up the idea of not only a red-light activated system, but also creating a signaling regulatory network as either a positive or negative feedback. This connects to our project because of the idea of increased signaling in a controlled system.

Plan for Future: After independent research on all of the parts, we can now join together the ideas into a cohesive whole in our plan for the iGEM project.