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Team:Stanford/Notebook/Lab Work/Week 5
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7/26 Monday
Laura's Notebook
helped Francisco aliquot competent cells (thaw main tube- 1 mL- on ice, transfer 50 uL to each of 20 pre-chilled 0.5 mL microfuge tubes)
7/27 Tuesday
Laura's notebook
redo failed ligation: vary vector/insert ratios
| prior recipe | ligation 1 | ligation 2 | ligation 3 | |
| water | none | 11.0 | 7.0 | none |
| vector | 5.0 | 2.0 | 2.0 | 2.0 |
| insert | 12.0 | 4.0 | 8.0 | 15.0 |
| 10X buffer | 2.0 | 2.0 | 2.0 | 2.0 |
| ligase | 1.0 | 1.0 | 1.0 | 1.0 |
- vector=B1006 linearized (cut with EcoRI, XbaI; inserts=GFP or RFP cut with EcoRI, SpeI (6 ligations set up)
- Francisco transformed these
7/28 Wednesday
Laura's Lab Notebook
miniprepped (ProMega kit): GFP and B1006 terminator (RFP didn't grow) NanoDrop data:
| sample | 260/280 | 260/230 | ng/uL |
| B1006 | 1.61 | 0.95 | 49.4 |
| GFP | 1.83 | 1.22 | 96.4 |
