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JULY: WEEK 4
July, 19th
TECAN test showed that no RFP was produced from our parts, so all parts are potentially correct! For this reason we decided to sequence:
- I14-1 (Forward)
- I16-1 (Forward)
- I17-1 (Forward)
- I18-1 (Forward)
- I19-1 (Forward)
We also sequenced:
- I74C5-2 (Forward and Reverse)
- I84C5-2 (Forward and Reverse)
- I12-2 (Forward and Reverse)
These samples were prepared for sequencing (DNA was essicated) and sent to BMR genomics.
Trasformation of RING into:
- BW25141 (pir+)
- BW25142 (pir116)
- BW23474 (pir116)
- DH5alpha
- MG1655
Cultures were plated on:
- BW2514: Cm 34ug/ml
- BW25142: Cm 34ug/ml
- BW234741: Cm 34ug/ml
- DH5alpha: Cm 12,5ug/ml
- MG1655: Cm 12,5ug/ml
July, 20th
 BW23474 transformed with <partinfo>BBa_J72007</partinfo> |  DH5alpha transformed with <partinfo>BBa_J72007</partinfo> |
Results for plates incubated ON, 37° C:
- BW23474 (pir116): showed colonies
- BW25141 (pir+): showed colonies
- BW25142 (pir116): showed colonies
- DH5alpha: didn't show colonies
- MG1655: didn't show colonies (even if there were a very few colonies that we suppose integrated the resistance of RING to survive - or the plate antibiotic wasn't homogeneous)
 BW23474 transformed with RING |  BW25141 transformed with RING |  BW25142 transformed with RING |
 DH5alpha transformed with RING |  MG1655 transformed with RING |
Single colonies were picked from plates and grown in LB+Cm at proper concentration. MG1655 colonies were let grow in LB+Cm to check if they integrated the Cm resistance of RING.
Inoculum of:
| I10-1 | I12-2 | I17-1
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| I14-1 | <partinfo>BBa_J23110</partinfo> | I3-1
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in 5ml LB+Amp. Cultures were grown ON 37°C 220 rpm.
July, 21st
July, 22nd
July, 23rd
July, 24th
July, 25th
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