Wednesday July 28, 2010

Patrick

The progress on learning Autodesk Maya seems to be going quite smoothly. I spent much of the day reading through the basic tutorials. I now know how to manipulate and create various polygonal and smooth-surface shapes. Tomorrow I will continue learning the program in polygonal modelling.

Chris

Today, I started the constructions of degP and CpxR promoters (K135000 and K239000) with the RFP and GFP constructs (I13507 and I13504) using the restriction enzymes XbaI, SpeI, and PstI. The constructions were done into AK plasmid backbones. As well, I started the PCR with primers that were previously designed by Alex and Patrick to take the CpxP promoter from the E. coli genome. The PCR finished but it ended too late to run on a gel. Tomorrow, that gel will be run. I continued contacting companies about possible sponsorships for our project. Our current sponsors will be posted on the website soon.