Chris's gel electrophoresis of the plasmid PCR of the CpxP promoters.

Tuesday July 27, 2010

Chris

Today, I ran a 1.0% agarose gel electrophoresis of the CpxP promotor PCR that was left to be done overnight.

Jeremy

Today I ran a 0.8% gel. Prepared overnights for the I0500+B0034 in AK ccdB.

Patrick

We have decided to start using Autodesk Maya to create an animation of inclusion body formation. This means I need to learn Autodesk Maya. Thus, I spent much of the day doing so. Jeremy and I also discussed the things we need the rest of the team to help contribute to the wiki.

Emily

Unfortunately the gel of my PCR to Biobrick malE and malE31 showed no amplification whatsoever so I have to strat this again. Today I did a gradient PCR to try to biobrick malEdelSS and malE31delSS. They are currently in the plasmids supplied to us by the Betton Labs. I also set up two more gradient PCRs to try to biobrick malE and malE31. I also set up a restriction digest with XbaI and SpeI of the PCR product from the attempt at biobricking malE and malE31 from last week. I left these to digest overnight along with psb1AC3 and psb1AK3 vectors in order to attempt to get biobricked malE and malE31 into the BBK vectors. I will transform these tomorrow and then plate them for overnight growth. I also looked into a paper on malE and the cpxP, cpxR and degP pathways.