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From 2010.igem.org

Lab Work

Pseudomonas Putida GPO1

The second day of the grow process of Pseudomonas Putida GPO1

Sample	medium	day 1 = 30 June	day 2= 1 July
no cells	E2 without carbon	0.00	0.00
no cells	LB	0.00	0.00
no cells	E2 + 1% LB	0.00	0.01
Pseudomonas Putida GPO1 *	E2 + 1% LB	0.01	0.05
no cells	E2 + 10% LB	0.00	0.00
Pseudomonas Putida GPO1 *	E2 + 10% LB	0.34	0.19
no cells	E2 + 1% octane	0.00	0.00
Pseudomonas Putida GPO1 *	E2 + 1% octane	0.00	0.00
Pseudomonas Putida GPO1 *	E2 + 10% octane	0.29	0.18

* average of 3 colonies

Pseudomonas Putida GPO1 can grow on 10% octane as carbon source.

Competent cells

Top10 cells were cultivated for making competent cells.